Description
A set of anti-protamine monoclonal antibodies (MAbs) that recognize and bind to isolated protamines P1 and/or P2 and to their native states (while bound to DNA in spermatids and sperm). Reference: Balhorn, R., Steger, K, Bergmann, M, Schuppe, H.C., Neuhauser, S. and Balhorn, MC (2018) Systems Biology in Reproductive Medicine, 64(6): 424-447. (pdf), Supplemental Information (Fig. S1), (Fig. S2), (Fig. S3).
Our products are for use in laboratory research only.
These MAbs have been tested for their selectivity in binding to protamines in four species (human, mouse, stallion and bull). However, the epitopes identified indicate these antibodies should recognize protamines present in the spermatids and sperm of a much broader range of species than Hup1N, Hup2b or Hup1M MAbs do.
Features that differ from the Hup MAbs include:
1) a broader range of mouse antibody isotype,
2) the recognition of different protamine P1 and P2 epitopes,
3) applicability for use in chromatin immunoprecipitation assays, and
4) other uses that require the recognition of protamines P1 or P2 from most mammalian species and many marsupials, birds and reptiles.
Please see Useful Information Page for Protocols. Note: Protamines are very basic proteins and are insoluble in SDS, so please use acid-urea gels and not SDS gels.
Characteristics of the new BPB protamine monoclonal antibodies
ELISA titration curves
comparing BPB MAbs with Hup MAbs
for antigen specificity in four species
ELISA tritation curves for binding of BPB MAbs to human, mouse, stallion, and bull protamine antigens
